Research

Biocatalytical processing

Conversion of lignocellulosic biomass to biofuels and chemicals offers decreased dependency on fossil sources, improved energy security, and a move from environmental challenges associated with petroleum. Two major constituents of lignocellulosic biomass, cellulose and hemicellulose, can be hydrolyzed to monosaccharide sugars that can be utilized for production of bioalcohols, carboxylic acid and other commodities in microbial fermentation processes. The binding of cellulose- binding domain (CBD) can thus significantly contribute to the activity of the cellulase against cellulosic substrates by increasing enzyme–substrate proximity, enhancing accessibility, and modifying the structure and surface of the cellulose, being of higher interest (Marie-Curie-ToK/DeV-FP6-POLYSURF). Production of bioactive molecules, chemicals, intermediates and/or additives is another aspect.

CIOLACU, Diana, CHIRIAC, Alina Iulia, PASTOR, F. I. Javier, KOKOL, Vanja. The influence of supramolecular structure of cellulose allomorphs on the interactions with cellulose-binding domain, CBD3b from Paenibacillus barcinonensis. Bioresource technology, ISSN 0960-8524. [Print ed.], Apr. 2014, vol. 157, 14-21. doi: 10.1016/j.biortech.2014.01.027

CIOLACU, Diana, GORGIEVA, Selestina, TAMPU, Daniel, KOKOL, Vanja. Enzymatic hydrolysis of different allomorphic forms of microcrystalline cellulose. Cellulose (Lond.), 2011, vol. 18, no. 6, 1527-1541, doi: 10.1007/s10570-011-9601-4.

CIOLACU, Diana, KOVAČ, Janez, KOKOL, Vanja. The effect of the cellulose-binding domain from Clostridium cellulovorans on the supramolecular structure of cellulose fibres. Carbohydr. res.. [Print ed.], Mar. 2010, vol. 345, iss. 5, 621-630, doi: 10.1016/j.carres.2009.12.023.

FILIPIČ, Jasmina, KRAIGHER, Barbara, TEPUŠ, Brigita, KOKOL, Vanja, MANDIĆ-MULEC, Ines. Effects of low-density static magnetic fields on the growth and activities of wastewater bacteria Escherichia coli and Pseudomonas putida. Bioresour. technol.. [Print ed.], Sep. 2012, vol. 120, 225-232, doi: 10.1016/j.biortech.2012.06.023.

BOŽIČ, Mojca, PRICELIUS, Sina, GÜBITZ, Georg M., KOKOL, Vanja. Enzymatic reduction of complex redox dyes using NADH-dependent reductase from Bacillus subtilis coupled with cofactor regeneration. Appl. microbiol. biotechnol., Jan. 2010, vol. 85, no. 3, 563-571, doi: 10.1007/s00253-009-2164-8.

BOŽIČ, Mojca, DÍAZ-GONZÁLEZ, María, TZANOV, Tzanko, GÜBITZ, Georg M., KOKOL, Vanja. Voltametric monitoring of enzyme-mediated indigo reduction in the presence of various fibre materials. Enzyme microb. technol.. [Print ed.], Oct. 2009, vol. 45, iss. 4, 317-323, doi: 10.1016/j.enzmictec.2009.05.009.

BOŽIČ, Mojca, KOKOL, Vanja, GÜBITZ, Georg M. Indigo dyeing of polyamide using enzymes for dye reduction. Tex. res. j., 2009, vol. 79, no. 10, 895-907, doi: 10.1177/0040517508097514.

BOŽIČ, Mojca, KOKOL, Vanja. Ecological alternatives to the reduction and oxidation processes in dyeing with vat and sulphur dyes. Dyes pigm.. [Print ed.], 2008, vol. 76, iss. 2, 299-309. http://dx.doi.org/10.1016/j.dyepig.2006.05.041.

KOKOL, Vanja, DOLIŠKA, Aleš, EICHLEROVÁ, Ivana, BALDRIAN, Petr, NERUD, František. Decolorization of textile dyes by whole cultures of Ischnoderma Resinosum and by purified laccase and Mn-peroxidase. Enzyme microb. technol.. [Print ed.], Jun. 2007, vol. 40, iss. 7, 1673-1677. http://dx.doi.org/10.1016/j.enzmictec.2006.08.015.

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