Research

Bioactive materials and coatings for tissue engineering

Biomedical scaffolds (EraNet-MNT-2013-nPOSSCOG) and coatings (Erasmus-Mundus-2013–EUPHRATES, ARRS-J3-9262, ARRS-J2-1719) used in tissue engineering should meet many different requirements, from biocompatibility to physic-mechanical properties and micro-to-nano porosity that direct tissue formation, support oxygen and nutrition diffusion toward the cells and drain waste products from the matrix. Both, the composition and pore interconnectivity are also essential for promotion of cell attachment, differentiation, proliferation and migration, thus regulating the cell’s behaviour, morphology and development (phenotype).. Biodegradability and non-cytotoxicity of degradation products are another issue that plays significant role in cell attachment, and bio-safety ECM replacement of the live tissue.

POTTA THARA, Yasir Beeran, KOKOL, Vanja. Effect of nozzle diameter and cross-linking on the micro-structure, compressive and biodegradation properties of 3D printed gelatin/collagen/hydroxyapatite hydrogel. Bioprinting. June 2023, vol. 31, e00266, 9 p. DOI: 10.1016/j.bprint.2023.e00266.

POTTA THARA, Yasir Beeran, JORDAN, Miha, GOMBOC, Timi, KAMENIK, Blaž, VIHAR, Boštjan, KOKOL, Vanja, ZADRAVEC, Matej. Solidification of gelatine hydrogels by using a cryoplatform and its validation through CFD approaches. Gels. June 2022, vol. 8, iss. 6, 17-. DOI: 10.3390/gels8060368.

KOKOL, Vanja, POTTA THARA, Yasir Beeran, MIHELČIČ, Mohor, SLEMENIK PERŠE, Lidija. Rheological properties of gelatine hydrogels affected by flow- and horizontally-induced cooling rates during 3D cryo-printing. Colloids and surfaces. A, Physicochemical and Engineering Aspects, 2021, vol. 616 (126356), 1-10, doi: 10.1016/j.colsurfa.2021.126356

POTTA THARA, Yasir Beeran, VUHERER, Tomaž, MAVER, Uroš, KOKOL, Vanja. Morphological, mechanical, and in-vitro bioactivity of gelatine/collagen/hydroxyapatite based scaffolds prepared by unidirectional freeze-casting. Polymer testing, 2021, vol. 102 (107308), 1-13, doi: 10.1016/j.polymertesting.2021.107308.

PAL, Snehashis, PERŠIN, Zdenka, VUHERER, Tomaž, DRSTVENŠEK, Igor, KOKOL, Vanja. The effect of Ti-6Al-4V alloy surface structure on the adhesion and morphology of unidirectional freeze-coated gelatin. Coatings, 2020, vol. 10, iss. 5 (434), 1-19, doi: 10.3390/coatings10050434.

INGOLE, Vijay H., GHULE, Shubham S., VUHERER, Tomaž, KOKOL, Vanja, GHULE, Anil V. Mechanical properties of differently nanostructured and high-pressure compressed hydroxyapatite-based materials for bone tissue regeneration: their origin, distribution and associations within hypersaline sediments. Minerals, 2021, vol. 11, iss. 12 (1390), 1-16, doi: 10.3390/min11121390.

INGOLE, Vijay H., VUHERER, Tomaž, MAVER, Uroš, VINCHURKAR, Aruna, GHULE, Anil V., KOKOL, Vanja. Mechanical properties and cytotoxicity of differently structured nanocellulose-hydroxyapatite based composites for bone regeneration application. Nanomaterials, 2020, vol. 10, iss. 1, 1-17, doi: 10.3390/nano10010025.

INGOLE, Vijay H., HUSSEIN, Kamal Hany, KASHALE, Anil A., GHULE, Kalyani, VUHERER, Tomaž, KOKOL, Vanja, CHANG, Jia-Yaw, LING, Yong-Chien, VINCHURKAR, Aruna, DHAKAL, Hom N., GHULE, Anil V. Ultrasound assisted green economic synthesis of hydroxyapatite nanoparticles using eggshell bio-waste and study of mechanical and biological properties for orthopaedic applications. Journal of biomedical materials research. Part A, 2017, vol. 105, iss. 11, 2935-2947, doi: 10.1002/jbm.a.36146.

TIYYAGURA, Hanuma Reddy, FUCHS-GODEC, Regina, GORGIEVA, Selestina, ARTHANARI, Srinivasan, MOHAN, Mantravadi Krishna, KOKOL, Vanja. Biomimetic gelatine coating for less-corrosive and surface bioactive Mg-9Al-1Zn alloys. Journal of materials research, 2018, vol. 33, iss. 10, 1449-1462, doi: 10.1557/jmr.2018.65.

TIYYAGURA, Hanuma Reddy, RUDOLF, Rebeka, GORGIEVA, Selestina, FUCHS-GODEC, Regina, VENKATAPPA RAO, Boyapati, MOHAN, Mantravadi Krishna, KOKOL, Vanja. The chitosan coating and processing effect on the physiological corrosion behaviour of porous magnesium monoliths. Progress in organic coatings, 2016, vol. 99, iss. 4, 147-156. doi: 10.1016/j.porgcoat.2016.05.019.

GORGIEVA, Selestina, GIRANDON, Lenart, KOKOL, Vanja. Mineralization potential of cellulose-nanofibrils reinforced gelatine scaffolds for promoted calcium deposition by mesenchymal stem cells. Materials science & engineering. C, Materials for biological applications, 2017, vol. 73, 478-489, doi: 10.1016/j.msec.2016.12.092.

LIŠKOVÁ, Jana, DOUGLAS, Timothy E. L., BERANOVÁ, Jana, SKWARCZYŃSKA, Agata, BOŽIČ, Mojca, KESHARI SAMAL, Sangram, MODRZEJEWSKA, Zofia, GORGIEVA, Selestina, KOKOL, Vanja, BAČÁKOVÁ, Lucie. Chitosan hydrogels enriched with polyphenols: antibacterial activity, cell adhesion and growth and mineralization. Carbohyd. Polym., 2015, vol. 129, 135-142, doi: 10.1016/j.carbpol.2015.04.043.

GORGIEVA, Selestina, KOKOL, Vanja. Bio mimetically engineered materials for more effective healthcare, International Conference LET’S 2014, Leading Enabiling Technologies for Societal Challenges, Sept. 29th – Oct. 1st 2014, Bologna, Italy. http://www.lets2014.eu/programme/posters-session/

PODLIPEC, Rok, GORGIEVA, Selestina, JURAŠIN, Darija, URBANČIČ, Iztok, KOKOL, Vanja, ŠTRANCAR, Janez. Molecular mobility of scaffolds` biopolymers influences cell growth. ACS applied materials & interfaces, Aug 2014, vol. 6, iss. 18, 15980-15990, doi: 10.1021/am5037719.

GORGIEVA, Selestina, KOKOL, Vanja. Processing of gelatin-based cryogels with improved thermo-mechanical resistance, pore size gradient and high potential for sustainable protein-drug release. Journal of biomedical materials research. Part A, 2015, vol. 103, iss. 3, 1119-1130, doi: 10.1002/jbm.a.35261.

GORGIEVA, Selestina, ŠTRANCAR, Janez, KOKOL, Vanja. Evaluation of surface/interface-related physico-chemical and micro-structural properties of gelatin 3D scaffolds, and their influence on fibroblast growth and morphology, Journal of biomedical materials research. Part A, 2014, vol. 102, iss. 11, 3986-3997, doi: 10.1002/jbm.a.35076.

GORGIEVA, Selestina, KOKOL, Vanja. Collagen- vs. gelatine-based biomaterials and their biocompatibility : review and perspectives. V: PIGNATELLO, Rosario (ur.). Biomaterials applications for nanomedicine. Rijeka: InTech, cop. 2011, 17-52. http://www.intechopen. com/articles/show/title/collagen-vs-gelatine-based-biomaterials-and-their-biocompatibility-review-and-perspectives.

MUSIAŁ, Witold, KOKOL, Vanja. Stimuli-sensitive polymer gels for dermal and transdermal drug delivery and their application in development of smart textile materials. V: FANUN, Monzer (ur.). Colloids in drug delivery, (Surfactant science series, v. 148). Boca Raton: Taylor & Francis, 2010, 137-155, graf. prikazi.

GORGIEVA, Selestina, KOKOL, Vanja. Preparation, characterization, and in vitro enzymatic degradation of chitosan-gelatine hydrogel scaffolds as potential biomaterials. J. biomed. mater. res., Part A, 2012, vol. 100A, iss. 7, 1655-1667, doi: 10.1002/jbm.a.34106.

GORGIEVA, Selestina, KOKOL, Vanja. Synthesis and application of new temperature-responsive hydrogels based on carboxymethyl and hydroxyethyl cellulose derivatives for the functional finishing of cotton knitwear. Carbohydr. polym., 2011, vol. 85, iss. 3, 664-673, doi: 10.1016/j.carbpol.2011.03.037.

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